Author(s): Mathew PieroNgugi, Oduor Richard Okoth, Omwoyo Richard Ombori, Njagi Joan Murugi, Mgutu Allan Jalemba and Cheruiyot Richard Chelule
A reproducible regeneration system based on direct somatic embryogenesis is described for Kenya cassava lines. Cassava plants were regenerated at high frequency by inducing shoot primordial on explants derived from cotyledons of cassava somatic embryos. Various parameters were evaluated on their effects on callus induction, somatic embryogenesis, maturation and germination of somatic embryos as well as recovery of regenerated plantlets. Immature leaf lobes were used as explants for somatic embryogenesis. Three Kenyan cassava genotypes viz; Adhiambo Lera, Kibanda Meno and Serere along with a model cultivar, TMS 60444 were used this system. Remarkable regeneration frequencies were observed in all the evaluated genotypes with Adhiambo Lera showing the best responses. As a result, a highly efficient plant regeneration protocol via germination of somatic embryos was achieved. This system enriches the scope of in vitro regeneration protocols for cassava and is envisaged to be a reliable prerequisite to genetic transformation of African cassava genotypes.
