Author(s): Spandana B, DSRS Prakash and Nagalakshmi S
By using Bioinformatic tools three sets of codehop, webprimer and manually, gene specific degenerate primers were designed for Sesamum desaturase. Through Gradient PCR approach maximum homology among desaturase sequences was observed to be with Arachis hypogaea than the other oil seed crops compared. Primers designed through Codehop and webprimershowed amplification of the gene sequence. The aim of this present study was to utilize bioinformatic tools for sesame improvement programs towards diversifying the fatty acid composition of the seed oil.
