Author(s): Mihiret Mekonnen, Ano Wariyo and Guddisa Hilu
Rosemary (Rosmarinus officinalis L.) is a common household aromatic and medicinal plant belonging to the Labiatae family and sage (Salvia officinalis) Lamiaceae family is aromatic, rather woody perennial low shrub. This study aimed some antifungal effects of plant essential oils were investigated against fungal disease agent Fusarium oxysporum on mycilial growth inhibition in vitro and disease development reserve in vivo conditions. The tissue pieces were aseptically transferred to petridishes containing a potato dextrose agar medium supplemented with streptomycin sulfate at the rate of three to five pieces per plate. Fusarium oxysporum was grown for 7-10 days on PDA at 27°C. The concentrations were tested at 20, 40, 60 and 80 μl. The same quantity of PDA Fusarium oxysporum also established in control. F. oxysporum was cut with a sterile cork borer from the edge of actively growing cultures on PDA plates. At 20 μl, 40 μl, 60 μl and 80 μl concentration, lemon grass (100%) growth inhibition was confirmed against Fusarium wilt followed by spearmint (85.00%) and minimum inhibition recorded 25.00% at Eucalyptus. As conclusion lemon grass and spear mint essential oils demonstrated the maximum antagonistic effect against the Fusarium wilt, where as Eucalyptus inhibit the list amount of growth pathogen.
